From Wikipedia,
the free encyclopedia.
Bimolecular fluorescence
complementation (BiFC)
is a method of viewing the
association of
proteins inside living
cells. Kerppola et al. (2002)
solidified this methodology as
viable
in vivo.
How it works
Two non-fluorescent fragments
of a
fluorescent protein are fused
(see
fused protein) to proteins of
interest. When the two proteins of
interest associate, the fragments
join and fluoresce. This
fluorescence is detected and
recorded via a
camera mounted on a
fluorescence microscope.
